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Seminars

Institute of Cell Biology Seminar Series

 

Seminars are held on Mondays at 12 noon or Wednesdays in May at 12 noon in Lecture Theatre 1, Daniel Rutherford Building, King's Buildings

Everyone is welcome to attend.

 

Semester Two:  January - June 2017

 

Date

Speaker

Host

9 Jan 2017

Stefan Westermann

University of Duisburg-Essen, Germany

Adele Marston

16 Jan 2017

Amy MacQueen

Wesleyan University, Connecticut, USA

Bill Earnshaw

23 Jan 2017

John Schwabe

University of Leicester

Adrian Bird

30 Jan 2017

Dirk Inzé

Ghent University, Belgium

Gerben van Ooijen

6 Feb 2017

Thornsten Allers

University of Nottingham

Sveta Makovets

13 Feb 2017

Erich Nigg

University of Basel, Switzerland

JP Arulanandam

20 Feb 2017

Philippe Pasero,

CNRS, Montpellier, France

Patrick Heun

27 Feb 2017

Patrick Schweizer,

Breeding Research, IPK Gatersleben, Germany

Gary Loake

6 March 2017

Anne Donaldson

University of Aberdeen

Sara Buonomo

13 March 2017

tbc

 

20 March 2017

Zuzana Storchova

MPIB, Munich, Germany

Adele Marston

27 March 2017

Peter Etchells

University of Durham

Naomi Nakayama

3 April 2017

Mariia Yuneva

The Francis Crick Institute

Sveta Makovets

10 April 2017

NO SEMINAR

 

17 April 2017

NO SEMINAR - EASTER MONDAY

 

24 April 2017

tbc

 

Wed 3 May 2017

WT PhD programme seminar - tbc

 

Wed 10 May 2017

WT PhD programme seminar - tbc

 

Wed 17 May 2017

WT PhD programme seminar - tbc

 

Wed 24 May 2017

WT PhD programme seminar - tbc

 

5 June 2017

Sue Biggins

Fred Hutchinson Cancer Research Center, Seattle, USA

Kevin Hardwick

12 June 2017

Alison Parkin

University of York

Louise Horsfall

19 June 2017

Matthieu Piel

Institut Curie, Paris, France

Julie Welburn

26 June 2017

tbc

 

 

Seminar details (including ad hoc seminars)

Date Event
28th Feb 2017
12:30
Michael Swann
7.20

Mohan Balasubramanian, Biomedical Sciences, University of Warwick

Cytokinesis in vitro and in vivo

Host: Ken Sawin


23rd Feb 2017
13:00
Michael Swann
7.20

Gavin Kelsey, Babraham Institute

Transcription as a driver of epigenetic transitions in the oocyte

Chromatin organisation is fundamental to genomic regulation, of which histone modifications are an integral part, however, it remains unclear to what extent histone modifications are an instructive component of the epigenetic landscape. The oocyte provides an attractive system to investigate temporal aspects of epigenetic regulation, because of the extensive epigenetic remodelling that occurs in a non-dividing cell; moreover, chromatin states in the oocyte may inform gene regulation in the zygote. Using new ultra low input chromatin immunoprecipitation methods to interrogate histone modifications throughout oogenesis, we observe widespread reprogramming of H3K4me3 in early oocytes, which subsequently accumulates independent of transcription. A consequence of the widespread deposition of H3K4me3 was the generation de novo of bivalent promoters and loss of canonical enhancer marks. Using conditional knock-outs to ablate H3K4me3 or DNA methylation in oocytes revealed, unexpectedly, that DNA methylation was dominant over H3K4me3 deposition. These results suggest that histone remodelling is not a driver of genomic regulation in the oocyte, but reflects transcriptional activity and targeting of DNA methylation.

Host: Adrian Bird


23rd Jan 2017
12:00
Daniel Rutherford
G.27, LT1

Professor John Schwabe - Department of Molecular and Cell Biology (formerly Biochemistry), Henry Wellcome Laboratories of Structural Biology,
 University of Leicester

Monday Seminar Series - "The molecular mechanisms of epigenetic transcriptional repression complexes"

My research group seeks to understand the molecular mechanisms that underlie the epigentic reprogramming of the genome during cellular differentiation and development. Our particular interest is in Histone Deacetylase (HDAC) complexes whose primary role is to remove acetyl groups from lysine sidechains within the tails of histone proteins resulting in hypo-acetylated chromatin that is associated with inactive or repressed genes.

There are at least five classes of HDAC-containing complexes that are targeted to chromatin. These contain the class I HDACs 1, 2 or 3 along with a variety of other proteins that target the complex to chromatin and/or possess other enzymatic activities. Importantly, the enzymatic activity of the HDACs requires assembly into these complexes.

Our structural and functional studies have given insight into the assembly and mechanism of action of these complexes. The crystal structure of HDAC3 in complex with its cognate co-repressors SMRT revealed, quite unexpectedly, that this complex is regulated by inositol phosphate. Studies of further HDAC complexes suggests that such regulation is a common theme. We are also exploring the assembly of several entire HDAC complexes and are beginning to understand their mode of assembly and the cross-talk between different components.

Host: Adrian Bird


19th Jan 2017
15:30
Michael Swann
7.20

Roy A Quinlan, University of Durham

Crafting "function" from "form" using cell proliferation and the cytoskeleton in the eye lens

The eye lens is a deceptively simple tissue, but it is a prime example of the D¹Arcy Thompson principle that "Form and Function” are linked. Our research addresses fundamental questions such as how do cells know their relative position in a tissue? What emergent properties are important for tissue formation? We believe that at least part of the answer to these questions lies in the lens epithelium. It is here that the iconic hexagonal shape of the lens fibre cells is established and the consequential spatial order established. During development this is easy to rationalize as the lens increases layer by layer onto a preformed template, but what happens when the lens regenerates? What determines the organization of the lens fibre cells in that scenario? We have built an interdisciplinary research team (John Girkin, Chris Saunter (Physics), Junjie Wu and Boguslaw Obara (SECS) with skills needed to study cell dynamics in the living zebrafish and in regenerating rat lenses. We have produced a mathematical model for the lens epithelium and we hope eventually to have a finite element model for lens accommodation. Along the way, we are studying the role of the intermediate filament cytoskeleton and their associated protein chaperones in maintaining lens optical functions, their effects upon cell shape and how ageing and disease affect their respective functions. The eye lens is a system that illuminates all the major key biological questions on ageing, cancer prevention, apoptosis as well as protein longevity. I shall select examples from my research portfolio to illustrate how form and function are linked so perfectly in the eye lens.

Host: Eric Schirmer


16th Jan 2017
12:00
Daniel Rutherford
G.27, LT1

Dr Amy MacQueen - Molecular Biology and Biochemistry, Wesleyan University

Monday Seminar Series - "Zippers and Stitches in the Meiotic Nucleus"

Our research seeks to understand the long mysterious but fundamental cellular mechanisms that drive chromosome dynamics during the differentiation of sex cells. A critical feature of sex cell differentiation is a reduction in chromosome number. During the specialized cell division cycle called meiosis, homologous partner chromosomes somehow identify and specifically associate with one another, and this association ensures their accurate segregation into separate chromosomal complements. A longstanding cell biological mystery is:  How does a chromosome search for and ultimately recognize its proper homologous partner in the nucleus? We use genetic, cytological, and biochemical approaches in conjunction with high resolution microscopy to identify the molecules and mechanisms that underlie how meiotic chromosomes interact, how homology detection occurs, and how homology recognition is coordinated with chromosome pairing reinforcement in the budding yeast, Saccharomyces cerevisiae.

 

            A transient yet dramatic reinforcement of initial homologous chromosome pairing occurs via the zipper-like assembly of a tripartite structure, the synaptonemal complex (SC), at the interface of lengthwise-aligned meiotic chromosomes. SC assembly between homologous chromosomes accompanies meiotic double strand break repair events at discrete sites along chromosomes; such homologous recombination events include crossovers (“stitches”), which splice DNA molecules of independent homologous chromosomes and serve to link homologs until their orientation and segregation on the meiosis I spindle. Past studies from our lab revealed several fundamental architectural and dynamic aspects of the budding yeast SC, as well as regulators that ensure SC assembles “at the right place, at the right time”. Our more recent studies have explored the functional relationship between the SC structure, its protein components, and meiotic recombination in budding yeast. Our observations suggest that many of the building block components of the budding yeast SC have distinct functions in meiotic recombination that may have evolved independently of their roles in SC assembly per se. An intriguing set of data indicates that an SC transverse filament protein, Zip1, may be mechanistically involved in crossover recombination in a manner that overlaps the activity of the MutS complex. Another observation reveals that the SC central element proteins, Ecm11 and Gmc2, promote proper mismatch repair during meiotic recombination. Our research is currently focused on experiments aimed at discovering the molecular features and underlying mechanisms that link such SC structural proteins to their essential roles in meiotic recombination.

Host: Bill Earnshaw


9th Jan 2017
12:00
Daniel Rutherford
G.27, LT1

Professor Stefan Westermann - Center of Medical Biotechnology, Department of Molecular Genetics, University of Duisburg-Essen

Monday Seminar Series - "Kinetochores and the microtubule cytoskeleton: Biochemical and genetic analysis of chromosome segregation in budding yeast"

Centromeres direct the assembly of kinetochores, microtubule-attachment sites thatallow chromosome segregation on the mitotic and meiotic spindle. I will discuss current projects in the lab that aim to elucidate the molecular organization of these chromosome segregation machines through a biochemical and genetic analysis of the budding yeast kinetochore. We have biochemically reconstituted large parts of the yeast kinetochore, including a full-length ten-protein KMN network. Current projects in the lab address how KMN is anchored to centromeric chromatin through association with multiple recruiters such as the CENP-C homolog Mif2, the AO complex and the CENP-T homolog Cnn1.

In addition, we are combining biochemical reconstitution experiments with single-molecule fluorescence microscopy to analyze mechanisms of microtubule-based motility. To this end we have performed a detailed analysis of the kinesin-14 protein Kar3, a minus-end directed molecular motor involved in microtubule organization and kinetochore transport. We are asking how the unusual biophysical properties of the Kar3 motor, whose movement depends on a non-catalytic domain, contribute to microtubule organization and kinetochore function.

Host: Adele Marston


23rd Nov 2016
15:30
Michael Swann Building
7.20

Mark C Field, University of Dundee

Evolution of the nuclear envelope - proteomics and comparative genomics to reconstruct the NPC and lamina

Host: Robin Allshire


22nd Nov 2016
13:00
Michael Swann Bulding
7.15

Andreas Ladurner, Ludwig-Maximilians-Universitat Munchen

Poly-ADP-ribose releases the autoinhibition of the oncogenic chromatin remodeler Alc1

Chromatin remodeling enzymes are essential in order to establish, alter or maintain eukaryotic chromatin structure and function. Their recruitment to the chromatin template is regulated by the presence of globular proteins domains capable of recognizing post-translationally modified histones, such as specific acetyl-lysine or methyl-lysine marks recognized by bromodomains and chromodomains, respectively. Increasing evidence, however, also suggests that post-translational modifications regulate the enzymatic activity of remodelers, and not just their recruitment. We will present unpublished work showing that the enzymatic activity of the human poly-ADP-ribose-dependent nucleosome remodeler Alc1/Chd1L is regulated exquisitely by oligomeric ADP-ribose ligands. Specifically, we find that tri-ADP-ribose binds the C-terminal macrodomain of the remodelers with nanomolar affinity, which in turns leads to a loss of intramolecular interaction with the catalytic Snf2 domain of the remodelers ATPase. ITC assays, MS-coupled H/D-exchange measurements, site-directed mutagenesis as well as real-time, live-cell imaging provide molecular insight into the allosteric activation of this remodeler mediated by oligomeric forms of ADP-ribose. Our data provide a mechanistic basis for Alc1/Chd1L’s function as an exquisite sensor of the DNA-damage-induced and PARP1-mediated synthesis of poly-ADP-ribose, fully consistent with the rapid recruitment of Alc1/Chd1L to DNA damage sites in live cells. Our data reveal an unprecedented mechanism for the ligand-induced, allosteric regulation of a critical remodeling enzyme and oncogene.

Host: Philipp Voigt and Atlanta Cook


17th Nov 2016
12:15
Michael Swann Building
7.20

Hiroshi Masumoto

Establishment and epigenetic maintenance of centromere chromatin on alpha-satellite DNA

Host: Bill Earnshaw


16th Jun 2015
10:00
Ashworth
Honours Room 1

Lawrence Dickson, Training and Audit Coordinator, University H&S Department

COSHH (Control of Substances Hazardous to Health) Training Talk, 10am to 11.00am

Host:


25th Jun 2014
16:45
Michael Swann Building
Main Lecture Theatre

Professor Caroline Dean - John Innes Centre, Norwich

Wellcome Trust PhD Programme in Cell Biology Symposium Lecture - Chromatin and antisense transcript dynamics underlying seasonal timing.

The study of adaptation in plants has led us into the dissection of conserved mechanisms that link antisense transcripts with chromatin dynamics. This work emerged from genetic and molecular analysis of flowering pathways that quantitatively modulate expression of the flowering repressor FLC in response to different environmental and developmental cues.

The antisense transcripts, collectively called COOLAIR, are alternatively spliced and polyadenylated, initiate in an R-loop covering the 3’ end of FLC and encompass the whole FLC sense transcription unit. The differentially processed forms of COOLAIR are associated with different FLC chromatin states via changes in recruitment of chromatin modifying complexes. The talk will describe our current understanding of these mechanisms.

Host: Wellcome Trust PhD Programme in Cell Biology