Mechanisms of epigenetic modifications
A schematic of the cycle of epigenetic modifications in the cell. Epigenetic modifications are initially deposited by writer enzymes. These are then read and interpreted in a co-operative manner by effector proteins. Epigenetic marks can also be removed by eraser proteins, which can reset the system. All modifications focus on the nucleosome hub, which can be modified on both the histone proteins and wrapped DNA. We are particularly interested in understanding how DNA damage repair and DNA methylation pathways are orchestrated by epigenetically-modified nucleosomes. Example Reader, writer and eraser proteins in these pathways are labelled. We hope to shed more light on how these proteins interact with epigenetic marks using biochemistry and structural biology approaches.
Wilson, M.D., Benlekbir, S., Fradet-Turcotte, A., Sherker, A., Julien, J.P., McEwan, A., Noordermeer, S.M., Sicheri, F., Rubinstein, J.L., and Durocher, D. (2016). The structural basis of modified nucleosome recognition by 53BP1. Nature 536, 100-103.
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