Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively.
Nowak JS, Hobor F, Downie Ruiz Velasco A, Choudhury NR, Heikel G, Kerr A, Ramos A, Michlewski G
Constitutive expression of untagged Lin28a affects the levels of numerous miRNAs during RA-induced neuronal differentiation of P19 cells. Due to its impaired function, the effects of the constitutive expression of GFP-tagged Lin28a are much more modest. Scatterplots of the fold change between day 0 and day 9 of the neuronal differentiation of P19 cells with GFP-tagged Lin28a and GFP only (left graph) and of P19 cells with untagged Lin28a and the control cell line (right graph). MiRNAs regulated more than twofold up or down by untagged Lin28a but not regulated by GFP-tagged Lin28a are highlighted with red circles.